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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
11/09/2013 |
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Autoria: |
LEMOS, N. G.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; MOLINA, J. C.; STOLF, R.; MORALES, A. M. R.; LUGLE, S. M.; SILVEIRA, C. A. de; MARIN, S. R. R.; BINNECK, E.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. |
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Título: |
Introduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 256. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
The soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More than 2590 embryos were transformed until now. Nineteen plants were PCR positives for the AHAS gene and none PCR positive plants were detected until now for the p35S:P5CS-129A construct. Number of insertion copies and expression levels will be tested in the positive plants. Physiological characterization in drought conditions will be carried on in promissing events. MenosThe soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More th... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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Marc: |
LEADER 03105naa a2200313 a 4500
001 1466831
005 2013-09-11
008 2004 bl uuuu u00u1 u #d
100 1 $aLEMOS, N. G.
245 $aIntroduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic.
260 $c2004
300 $ap. 256.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aThe soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More than 2590 embryos were transformed until now. Nineteen plants were PCR positives for the AHAS gene and none PCR positive plants were detected until now for the p35S:P5CS-129A construct. Number of insertion copies and expression levels will be tested in the positive plants. Physiological characterization in drought conditions will be carried on in promissing events.
650 $aSoja
700 1 $aYAMAGUCHI-SHINOZAKI, K.
700 1 $aNAKASHIMA, K.
700 1 $aMOLINA, J. C.
700 1 $aSTOLF, R.
700 1 $aMORALES, A. M. R.
700 1 $aLUGLE, S. M.
700 1 $aSILVEIRA, C. A. de
700 1 $aMARIN, S. R. R.
700 1 $aBINNECK, E.
700 1 $aFARIAS, J. R. B.
700 1 $aNEUMAIER, N.
700 1 $aNEPOMUCENO, A. L.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 10 | |
| 1. |  | OYA, T.; MARTINS, P. K.; CASAGRANDE, E. C.; LUGLE, S. M.; NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B. Agronomic, physiological and molecular characteristics of drought-tolerant Brazilian soybean cultivars. In: EMBRAPA SOYBEAN/JICA-CETAPAR/INTA-EEA/JIRCAS JOINT WORKSHOP ON SOYBEAN IMPROVEMENT, PRODUCTION AND UTILIZATION IN SOUTH AMERICA, 2001, Londrina. Abstracts and program. Londrina: Embrapa Soybean: JIRCAS, 2001. p. 17. (Embrapa Soja. Documentos, 170).| Biblioteca(s): Embrapa Soja. |
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| 2. | | PAIVA, A. A. R.; YAMAGUCHI-SHINOZAKI, K.; YAMANAKA, N.; NAKASHIMA, K.; FARIAS, J. R. B.; MARIN, S. R. R.; SILVEIRA, C. A.; LUGLE, S. M.; BENEVENTI, M. A.; ABDELNOOR, R. V.; POLIZEL, A. M.; NEPOMUCENO, A. L. Análise da indução do promotor rd29A de Arabidopsis thaliana em soja após déficit hídrico através de ensaio histoquímico e fluorimétrico em plantas transformadas com a construção rd29A:GUS. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 46-47. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 3. | | ROLLA, A. A. de P.; YAMAGUCHI-SHINOZAKI, K.; YAMANAKA, N.; NAKASHIMA, K.; FARIAS, J. R. B.; MARIN, S. R. R.; SILVEIRA, C. A.; LUGLE, S. M.; BENEVENTI, M. A.; ABDELNOOR, R. V.; POLIZEL, A. M.; NEPOMUCENO, A. L. Análise da indução do promotor rd29A de Arabidopsis thaliana em soja após déficit hídrico, através de ensaios histoquímico e fluorimétrico em plantas transformadas com a construção rd29A:GUS. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2., 2006, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2006. p. 171-174. (Embrapa Soja. Documentos, 276).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 4. | | BENEVENTI, M. A.; NEPOMUCENO, A. L.; YAMANAKA, N.; SHINOZAKI, K. Y.; NAKASHIMA, K.; BINNECK, E.; FARIAS, J. R. B.; MARIN, S. R. R.; SILVEIRA, C. A.; LUGLE, S. M.; ROLLA, A. A. P.; POLIZEL, A. M. Transformação genética em soja (Glycine max (L)Merril) visando tolerância à seca. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia. Resumos... Águas de Lindóia: SBG, 2005. 1 CD-ROM. Seção: Genética e Melhoramento - Resumos: Pdf.634.| Biblioteca(s): Embrapa Soja. |
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| 5. | | BENEVENTI, M. A.; NEPOMUCENO, A. L.; YAMANAKA, N.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; BINNECK, E.; FARIAS, J. R. B.; MARIN, S. R. R.; SILVEIRA, C. A.; PAIVA, A. A. R.; ABDELNOOR, R. V.; POLIZEL, A. M.; LUGLE, S. M. Transformação genética em soja pela inserção da construção gênica contendo a região promotora do gene rd29A e a região codante do gene DREB1A de Arabidopsis thaliana, visando tolerância à seca. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 47. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 6. | | BENEVENTI, M. A.; NEPOMUCENO, A. L.; YAMANAKA, N.; SHINOZAKI, K. Y.; NAKASHIMA, K.; BINNECK, E.; FARIAS, J. R. B.; MARIN, S. R. R.; SILVEIRA, C. A.; LUGLE, S. M.; ABDELNOOR, R. V.; ROLLA, A. A. P.; POLIZEL, A. M. Transformação genética de soja com promotor e fator de transcrição estresse induzidos visando tolerancia à seca. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005. p. 108-109. (Embrapa Soja. Documentos, 257). Organizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 7. | | NEPOMUCENO, A. L.; BENEVENTI, M. A., YAMAGUCHI-SHINOZAKI, K.; YAMANAKA, N.; NAKASHIMA, K.; BINNECK, E.; FARIAS, J. R. B.; MARIN, S. R. R.; SILVEIRA, C. A.; LUGLE, S. M.; ABDELNOOR, R.; PAIVA, A. A. R.; POLIZEL, A. M. Transformação genética em soja visando tolerância à seca. In: CONGRESO DE SOJA DEL MERCOSUR, 3., 2006, Rosário. Mercosoja 2006: mesas científico-técnicas, resúmenes expandidos / comunicaciones. Rosário: Associación de la Cadena de Soja Argentina, 2006. p. 242-245. Nome correto do décimo primeiro autor ABDELNOOR, R. V.| Biblioteca(s): Embrapa Soja. |
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| 8. | | NEPOMUCENO, A. L.; LUGLE, S. M.; ARIAS, C. A. A.; BRIGHENTI, A. M.; NEUMAIER, N.; FARIAS, J. R. B.; MARIN, S. R. R.; SILVEIRA, C. A.; BINNECK, E.; MOLINA, J.; LEMOS, N. G.; STOLF, R. Transformação de plantas de soja por biobalística com genes de interesse (04.2000.322-05). In: HOFFMANN-CAMPO, C. B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2002: ecofisiologia e biologia molecular. Londrina: Embrapa Soja, 2003. p. 39-46. (Embrapa Soja. Documentos, 217).| Biblioteca(s): Embrapa Soja. |
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| 9. | | ROLLA, A. A. de P.; NEPOMUCENO, A. L.; YAMANAKA, N.; SHINOZAKI, K. Y.; NAKASHIMA, K.; BINNECK, E.; FARIAS, J. R. B.; BENEVENTI, M. A.; SILVEIRA, C. A.; MARIN, S. R. R.; POLIZEL, A. M.; LUGLE, S. M. Obtenção de soja geneticamente modificada por biobalística visando tolerância à seca. In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA UNIFIL, 13., 2005, Londrina. Resumos... Londrina: UniFil, 2005. 1 CD-ROM. Área Biologia.| Biblioteca(s): Embrapa Soja. |
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| 10. | | LEMOS, N. G.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; MOLINA, J. C.; STOLF, R.; MORALES, A. M. R.; LUGLE, S. M.; SILVEIRA, C. A. de; MARIN, S. R. R.; BINNECK, E.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. Introduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 256. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 10 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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